Description
Clinically, mouse monoclonal antibodies (IgG) and their fragments are used in vivo diagnosis procedure (radionuclides) and treatment for patients with various diseases. In patients, even a single dose injection of murine monoclonal IgG may induce immune response directed against this foreign protein (immunogen). In the circulation, the presence of human antibody against murine IgG would bind to the injected murine IgG and, therefore, diminish the efficacy of either in-vivo diagnosis or treatment. Especially, the HAMA would increase the risk of anaphylactic complications to subsequent administration of the murine IgG based therapy. The presence of HAMA in patient serum or plasma specimens causes both false positive and false negative immunoassay test results depending on assay principles and monoclonal antibodies used in the assay system. This HAMA ELISA is a ready-to-use test kit with well-breakable microtiter plate and simple test procedures. It also provides a wide measurement range without high dose "hook" effect